BlueFISH Confirmatory Probes
The BlueGnome CytoChip uses arrayCGH to perform a genome wide
screen for regions of genomic copy number variation (CNVs) associated
with constitutional genetic disorders. In order to verify the physical
location of CNVs and confirm whether they are de novo or inherited
it is usual that all CNVs with a suspected phenotypic association be
validated using fluorescent in-situ hybridization (FISH).
To enable rapid FISH-validation of CNVs all the bacterial artificial
chromosome (BAC) clones on the CytoChip are available as BlueFISH
probes, a comprehensive library of 4400 high quality, pre-labelled,
FISH probes spanning the entire genome (research use only).
Accurate confirmation of CytoChip results.
By using the same BAC clones that are
used in the production of the CytoChip BlueFISH probes ensure consistency between
the array result and the FISH confirmation. This increases both the speed and
confidence of the confirmation.
Efficient ordering.
BlueFISH probes may be ordered in bulk and
then called off as required using comprehensive on-line ordering facilities
which are fully integrated with BlueGnome's arrayCGH analysis software,
BlueFuse for Microarrays. All probes required to confirm, report and close
the investigation are ordered in minutes and delivered in days.
Wider coverage.
BlueFISH probes cover the entire genome
with increased tiling on regions of disease interest.
Easier interpretation
Direct labelling and simple protocols deliver high signal
and low background for rapid, sensitive and specific confirmation of CNVs in both
metaphase chromosomes and interphase nuclei.
